Category: Methylene Blue

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• Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways – PubMed (nih.gov)
• Late-onset Alzheimer’s disease is associated with inherent changes in bioenergetics profiles | Scientific Reports (nature.com)
• Multimodal Randomized Functional MR Imaging of the Effects of Methylene Blue in the Human Brain | Radiology (rsna.org)
• Methylene Blue Shows Promise for Improving Short-Term Memory (rsna.org)
• View of A cohort of cancer patients with no reported cases of SARS-CoV-2 infection: the possible preventive role of Methylene Blue | Substantia (fupress.net)
• A controlled trial of methylene blue in severe depressive illness – PubMed (nih.gov)
• Mitochondrial Dysfunction in Autism | Autism Spectrum Disorders | JAMA | JAMA Network
• Methylene Blue Inhibits the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction-a Mechanism that can Contribute to its Antiviral Activity Against COVID-19
• Study finds methylene blue is a low-micromolar inhibitor of the the SARS-CoV-2 spike protein and ACE2 interaction
• Ueber schmerzstillende Wirkung des Methylenblaus (pp.493-494). von Ehrlich, Paul & Leppmann, A.: (1890) | Antiq. F.-D. Söhn – Medicusbooks.Com (zvab.com)
• Antifungal Action of Methylene Blue Involves Mitochondrial Dysfunction and Disruption of Redox and Membrane Homeostasis in C. albicans – PMC (nih.gov)
• Methylene Blue Protects Astrocytes against Glucose Oxygen Deprivation by Improving Cellular Respiration – PMC (nih.gov)
• Induced Oxidations in Blood. Hemoglobin Destruction by Methylene Blue in Lactic Acid Peroxidation – W. B. Wendel, 1930 (sagepub.com)
• Intraperitoneal administration of methylene blue attenuates oxidative stress, increases peritoneal fibrinolysis, and inhibits intraabdominal adhesion formation – PubMed (nih.gov)
• Methylene Blue to Extend Your Health Span (physicianspreferencerx.com)
• From Mitochondrial Function to Neuroprotection – An Emerging Role for Methylene Blue – PMC (nih.gov)
• Frontiers | Methylene Blue Application to Lessen Pain: Its Analgesic Effect and Mechanism (frontiersin.org)
• Methylene Blue has a potent antiviral activity against SARS-CoV-2 and H1N1 influenza virus in the absence of UV-activation in vitro | Scientific Reports (nature.com)
• Methylene Blue for Vasoplegic Syndrome after Cardiac Surgery: Early Administration Improves Survival – PMC (nih.gov)
• Methylene blue counteracts cyanide cardiotoxicity: cellular mechanisms | Journal of Applied Physiology
• Methylene Blue as an Antidote to CO Poisoning | American Journal of Physiology-Legacy Content
• Methylene Blue in the Treatment of Chronic Lyme Disease (rhealthc.com)
• Methylene blue adjuvant therapy of schizophrenia – PubMed (nih.gov)
• Early adjunctive methylene blue in patients with septic shock: a randomized controlled trial

• Drug interactions: Concurrent use of methylene blue with SSRIs, SNRIs, MAOIs, and other products which increase serotonin levels in the brain should be used with caution. Concurrent use, however, is not a contraindication. Consult your physican

• Monitor and avoid use in patients with G6PD deficiency (hemolysis).

• Do not use methylene blue in patients who are pregnant or breastfeeding due to lack of data.

• Side effects are rare when dosed under 2mg/kg per day and generally include aquamarine-colored urine roughly 4–12 hours following oral administration. Aquamarine-clored urine is normal when using methylene blue. Methylene blue at low doses selectively promotes mitochondrial respiration and cellular energy production. Long-term mitochondrial optimization is crucial for maintaining high levels of energy, oxygen transport, and protection against common pathogens, chronic inflammation, and premature cellular senescence. Methylene blue offers many opportunities to improve patient outcomes while reducing polypharmacy and should be strongly considered as a part of your health span extension protocol.

• For a therapeutic/nootropic regimen. A normal drop from a standard 30ml dropper bottle will contain approximately 0.05ml.
• For a nootropic regimen: .25ml – .50ml (2) times per day or 5 – 10 drops (2) times per day.
• Mix the drops in 8 oz (1 cup) of water. Please note that your tongue/mouth will temporarily stain blue and your urine will turn greenish/blue. This is normal.

For most indications, dosing .25ml – .50ml no more than 2 times daily is sufficient to improve health. Methylene blue is excreted in urine between 4-24 hours after administration with a half-life of 5-6.5 hours, so you can space out the doses according to your own schedule. Of course, if you are treating a specific problem, your doctor/researcher will guide you to a specific dosage.